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anti-cx3cr1 antibody  (Alomone Labs)


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    Alomone Labs anti-cx3cr1 antibody
    Anti Cx3cr1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-cx3cr1 antibody/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    anti-cx3cr1 antibody - by Bioz Stars, 2026-02
    93/100 stars

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    anti-cx3cr1 antibody  (Alomone Labs)
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    Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after <t>P2X4R</t> agonist ATP (1 μM) induced [Ca2+]i response. Calculated IC50 was 3.8 × 10−6 M.
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    Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after P2X4R agonist ATP (1 μM) induced [Ca2+]i response. Calculated IC50 was 3.8 × 10−6 M.

    Journal: Experimental neurology

    Article Title: Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke

    doi: 10.1016/j.expneurol.2020.113308

    Figure Lengend Snippet: Plot indicates the effect of 5-BDBD at various concentrations (10 nM to 30 μM; log10 [nM] = −4.52 to −8) after P2X4R agonist ATP (1 μM) induced [Ca2+]i response. Calculated IC50 was 3.8 × 10−6 M.

    Article Snippet: Briefly, all the cells isolated from the interphase 70%/30% Percoll gradient were washed and blocked with mouse Fc Block (clone 93, eBioscience, Thermo Fisher, Waltham, MA) before staining for 30 min in dark and cold conditions with primary antibody-conjugated fluorophores: CD45-eF450 (clone 2D1, eBioscience), CD11b-APCeF780 (clone M1/70, eBioscience), Ly6G-AF700 (clone 1A8, eBioscience), Ly6C-PerCP-Cy5.5 (clone HK1.4, BioLegend, San Diego, CA), CX3CR1 APC (Clone SA011F11, BioLegend), and P2X4R-FITC (AFR-024-F, Alomone Labs, Jerusalem, Israel).

    Techniques:

    Representative western blots are shown in the top panel. Effects of vehicle or 5-BDBD on pro and mature BDNF at (A) 3 days and (B) 30 days after stroke. C) Effects of vehicle and 5-BDBD treatment on pro/mature BDNF ratio 3 and 30 days after stroke. (D) P2X4R expression at 3 and 30 days after stroke were not different; Data are Mean ± SD (n=4–8 mice/group/time point). *p<0.05 for 5-BDBD vs vehicle by Student’s t-test. β-actin was used as internal control to normalize the data.

    Journal: Experimental neurology

    Article Title: Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke

    doi: 10.1016/j.expneurol.2020.113308

    Figure Lengend Snippet: Representative western blots are shown in the top panel. Effects of vehicle or 5-BDBD on pro and mature BDNF at (A) 3 days and (B) 30 days after stroke. C) Effects of vehicle and 5-BDBD treatment on pro/mature BDNF ratio 3 and 30 days after stroke. (D) P2X4R expression at 3 and 30 days after stroke were not different; Data are Mean ± SD (n=4–8 mice/group/time point). *p<0.05 for 5-BDBD vs vehicle by Student’s t-test. β-actin was used as internal control to normalize the data.

    Article Snippet: Briefly, all the cells isolated from the interphase 70%/30% Percoll gradient were washed and blocked with mouse Fc Block (clone 93, eBioscience, Thermo Fisher, Waltham, MA) before staining for 30 min in dark and cold conditions with primary antibody-conjugated fluorophores: CD45-eF450 (clone 2D1, eBioscience), CD11b-APCeF780 (clone M1/70, eBioscience), Ly6G-AF700 (clone 1A8, eBioscience), Ly6C-PerCP-Cy5.5 (clone HK1.4, BioLegend, San Diego, CA), CX3CR1 APC (Clone SA011F11, BioLegend), and P2X4R-FITC (AFR-024-F, Alomone Labs, Jerusalem, Israel).

    Techniques: Western Blot, Expressing, Control

    Images (top) and quantification (bottom) of P2X4R expression (green) in lba-1 positive cells (red) from immunostaining of brain sections 3 days after stroke. 20x magnification. DAPI (blue) = 4′,6-diamidino-2-phenylindole (nuclear stain). Scale bar 20 μm. Data are Mean ± SD (n=3 mice/group). *p<0.05, 5-BDBD vs. Vehicle; Student’s t-test.

    Journal: Experimental neurology

    Article Title: Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke

    doi: 10.1016/j.expneurol.2020.113308

    Figure Lengend Snippet: Images (top) and quantification (bottom) of P2X4R expression (green) in lba-1 positive cells (red) from immunostaining of brain sections 3 days after stroke. 20x magnification. DAPI (blue) = 4′,6-diamidino-2-phenylindole (nuclear stain). Scale bar 20 μm. Data are Mean ± SD (n=3 mice/group). *p<0.05, 5-BDBD vs. Vehicle; Student’s t-test.

    Article Snippet: Briefly, all the cells isolated from the interphase 70%/30% Percoll gradient were washed and blocked with mouse Fc Block (clone 93, eBioscience, Thermo Fisher, Waltham, MA) before staining for 30 min in dark and cold conditions with primary antibody-conjugated fluorophores: CD45-eF450 (clone 2D1, eBioscience), CD11b-APCeF780 (clone M1/70, eBioscience), Ly6G-AF700 (clone 1A8, eBioscience), Ly6C-PerCP-Cy5.5 (clone HK1.4, BioLegend, San Diego, CA), CX3CR1 APC (Clone SA011F11, BioLegend), and P2X4R-FITC (AFR-024-F, Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Immunostaining, Staining

    Flow-sorted microglia and monocytes cells from Fig 5 were fixed (2% paraformaldehyde) and analyzed by an ImageStream cytometer. Using the IDEAS software package, all doublets or aggregated cells were excluded by gating on single cells. A) P2X4R and CD11b+ were focused and selected. Images of single cells (~300 cells/sample, N=3 per group) of the bright field and fluorescent channel (P2X4R-FITC and CD11b-APC-eFluor780) were acquired, and overlays were generated. Magnification=60x. B) Ratio of median fluorescence intensity (MFI) of P2X4R/CD11b was significantly reduced after 5-BDBD treatment in both microglia and monocytes. Further, a separate comparison of microglia vs. monocytes P2X4R MFI suggests reduced expression of P2X4R in microglia irrespective of treatment. Data are Mean ± SD; *p<0.05, 5-BDBD vs. Vehicle; #p<0.05, microglia vs. monocytes; Student’s t-test.

    Journal: Experimental neurology

    Article Title: Neuroprotective and Neuro-rehabilitative Effects of Acute Purinergic Receptor P2X4 (P2X4R) Blockade after Ischemic Stroke

    doi: 10.1016/j.expneurol.2020.113308

    Figure Lengend Snippet: Flow-sorted microglia and monocytes cells from Fig 5 were fixed (2% paraformaldehyde) and analyzed by an ImageStream cytometer. Using the IDEAS software package, all doublets or aggregated cells were excluded by gating on single cells. A) P2X4R and CD11b+ were focused and selected. Images of single cells (~300 cells/sample, N=3 per group) of the bright field and fluorescent channel (P2X4R-FITC and CD11b-APC-eFluor780) were acquired, and overlays were generated. Magnification=60x. B) Ratio of median fluorescence intensity (MFI) of P2X4R/CD11b was significantly reduced after 5-BDBD treatment in both microglia and monocytes. Further, a separate comparison of microglia vs. monocytes P2X4R MFI suggests reduced expression of P2X4R in microglia irrespective of treatment. Data are Mean ± SD; *p<0.05, 5-BDBD vs. Vehicle; #p<0.05, microglia vs. monocytes; Student’s t-test.

    Article Snippet: Briefly, all the cells isolated from the interphase 70%/30% Percoll gradient were washed and blocked with mouse Fc Block (clone 93, eBioscience, Thermo Fisher, Waltham, MA) before staining for 30 min in dark and cold conditions with primary antibody-conjugated fluorophores: CD45-eF450 (clone 2D1, eBioscience), CD11b-APCeF780 (clone M1/70, eBioscience), Ly6G-AF700 (clone 1A8, eBioscience), Ly6C-PerCP-Cy5.5 (clone HK1.4, BioLegend, San Diego, CA), CX3CR1 APC (Clone SA011F11, BioLegend), and P2X4R-FITC (AFR-024-F, Alomone Labs, Jerusalem, Israel).

    Techniques: Cytometry, Software, Generated, Fluorescence, Comparison, Expressing